Antifade Mounting Solution / Mountant / Slide Mounting Medium for Fluorochromes


Glycerol (a non-fluorescent grade) is somewhat effective as an antifade solution if nothing else is available, although fluorochromes will fade rapidly.

0.5 % p-phenylenediamine (PPD)  (Free Base, Sigma) made up in 20 mM Tris, pH 8.8 with 90 % glycerol (non-fluorescent) is a good antifade mountant with most fluorochromes. Store at 4C in the dark. It will go dark brown with time, but this more offends the eye than reduces the effectiveness it seems.

Otherwise, commercial antifade solutions such as Citifluor, VectorShield, or ones from Molecular Probes can be used. Not different fluorochromes respond differently to various antifades (see for some parameters).