PREPARATION OF 3-AMINOPROPYLTRIETHOXYSILANE (APS, AES, APES or SILANE) TREATED SLIDES FOR ADHESION OF DNA (e.g. IN MICROARRAYS) OR TISSUE ADHESION
- Clean slides following protocol 5.1 in Practical In situ Hybridization (i.e. Soak in Chromic Acid solution for at least 4 hours, wash well in running water, then in distilled water and ethanol, oven dry).
(In fact, slides following this pretreatment bind chromosomes, extended DNA fibres (DNA fibers), and DNA in solution for microarrays quite effectively.)
- Immerse slides in a freshly prepared 2% solution for 3-aminopropyltriethoxysilane (e.g. Sigma A3648) in dry acetone for 30 seconds. Note 1.
- Wash twice in distilled water
- Dry overnight at 37C and store coated slides at room temperature (indefinitely; we have used 1 year without problems).
Note 1: Use a glass Coplin jar reserved exclusively for this purpose. The solution does not keep, but many slides can be coated at one time. Use in Fume Hood.