Recombination analysis around MS32

Single nucleotide and indel polymorphisms in a 206 kb interval surrounding minisatellite MS32 on chromosome 1q42.3

The location of each SNP (centromere to telomere) used in the linkage disequilibrium and sperm crossover studies is given relative to the position of the centre of minisatellite MS32 located at position 233,301,691 in Release 23.34e.1 of human chromosome 1. SNPs marked $ were initially discovered by resequencing 6-8 north Europeans. SNPs indicated by * were subsequently identified by haplotype sequencing around the MSTM1a and 1b hotspots and were not used for genotyping. Remaining SNPs were initially identified from the Public SNP Database. SNPs are named according to their position relative to MS32, except for SNPs A2 - Bl near MS32, some of which have been described previously (Jeffreys et al. Mol. Cell 2, 267-273, 1998).

Allele frequencies were determined from genotypes of a panel of 80 unrelated UK semen donors of north European origin. Genotypes were established by amplifying genomic DNA using Multiple Displacement Amplification (MDA) followed by hybridisation of appropriate 1-8 kb MDA PCR products with each of the indicated allele-specific oligonucleotides. For SNPs marked *, allele frequencies were estimated from haplotypes established from 26 semen donors. The SNP or indel site in each ASO is indicated in red. Mixed bases shown in green correspond to definite or suspected nearby SNP sites. Alu repeat sequences are shown in blue.

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SNP location,bp allele freq. allele-specific oligonucleotide

M-167.7 -167696 A 0.86 5' GGATGGAAAGTACTTCCA 3'

T 0.14 5' GGATGGATAGTACTTCCA 3'

M-167.2 -167188 A 0.26 5' ATGGCATAATCTCGGCTC 3'

G 0.74 5' ATGGCATGATCTCGGCTC 3'

M-167.1 -167127 C 0.26 5' AGCCTCCCGAGTAGCTTG 3'

T 0.74 5' AGCCTCCTGAGTAGCTTG 3'

M-166.5 -166455 A 0.12 5' AGAGGACAGTCCGTTCAG 3'

G 0.88 5' AGAGGACGGTCCGTTCAG 3'

M-152.4 -152372 C 0.31 5' TGGGACCCCCTGCGTTCC 3'

T 0.69 5' TGGGACCTCCTGCGTTCC 3'

M-148.8 -148770 T 0.82 5' GCTAAAATTGCCAACTCA 3'

C 0.18 5' GCTAAAACTGCCAACTCA 3'

M-138.3 -138296 T 0.81 5' GTGCAGTGGCTCATGCCT 3'

C 0.19 5' GTGCAGTGGCTCACGCCT 3'

M-129.7 -129655 G 0.77 5' GCCGGGCGCGGTGGCTCA 3'

A 0.23 5' GCCGGGCACGGTGGCTCA 3'

M-128.3 -128265 C 0.77 5' ACGGGGGCGGTCTCTTTG 3'

A 0.22 5' ACGGGGGAGGTCTCTTTG 3'

M-127.9 $ -127915 A 0.78 5' ATACCATATGCCACAGGG 3'

C 0.22 5' ATACCATCTGCCACAGGG 3'

M-126.7 $ -126676 - 0.78 5' AATTGTTGAACCCGGGAG 3'

+ 0.22 5' AATTGCTTGAACCCGGGA 3'

M-126.2 $ -126197 C 0.82 5' CAACTTTCTAAATGAAAA 3'

T 0.18 5' CAACTTTTTAAATGAAAA 3'

M-126.1 $ -126135 C 0.79 5' AAATTTTCATTTAAGGTA 3'

T 0.21 5' AAATTTTTATTTAAGGTA 3'

M-125.0 -125046 T 0.73 5' TGGCGGATGAGAAAAAGG 3'

A 0.27 5' TGGCGGAAGAGAAAAAGG 3'

M-124.9 -124861 T 0.74 5' GTTTAGCTTAAAGCTACT 3'

C 0.26 5' GTTTAGCCTAAAGCTACT 3'

M-123.1 $ -123134 + 0.79 5' TGTTTTGATAAAAGCCAT 3'

- 0.21 5' GTTTTGATATAAAGCCAT 3'

M-122.5 -122475 A 0.79 5' TTCCCTAAGATCCTGAAT 3'

G 0.21 5' TTCCCTAGGATCCTGAAT 3'

M-121.4a -121401 A 0.79 5' TTTTTTAAAAAAAGTTTA 3'

T 0.21 5' TTTTTTTAAAAAAGTTTA 3'

M-120.7 -120725 C 0.79 5' ATCTCGACTCCTTTATCC 3'

T 0.21 5' ATCTCGATTCCTTTATCC 3'

M-120.6 -120648 T 0.79 5' TTAGGACTCTGGGAAGCA 3'

G 0.21 5' TTAGGACGCTGGGAAGCA 3'

M-120.3 -120276 A 0.79 5' TCAGTGCAAGCAGTGGGG 3'

C 0.21 5' TCAGTGCCAGCAGTGGGG 3'

M-119.8a -119803 G 0.79 5' AGGCCNCGGCTCATAGGT 3'

A 0.21 5' AGGCCNCAGCTCATAGGT 3'

M-119.4 -119368 T 0.79 5' TCATTTCTGTTCGATACA 3'

C 0.21 5' TCATTTCCGTTCGATACA 3'

M-118.8 -118807 G 0.45 5' GGGCCAGGCTGCCTGGAC 3'

A 0.55 5' GGGCCAGACTGCCTGGAC 3'

M-118.0 -117977 T 0.22 5' GGGTGGGTCAAGSCTGCA 3'

C 0.77 5' GGGTGGGCCAAGSCTGCA 3'

M-118.0a -117972 C 0.79 5' GGYCAAGCCTGCATTTCT 3'

G 0.21 5' GGYCAAGGCTGCATTTCT 3'

M-116.3 -116281 T 0.79 5' ATAAATATATGGAATTCC 3'

C 0.21 5' ATAAATACATGGAATTCC 3'

M-115.8 -115788 C 0.79 5' CATTGTACCGTGGATATC 3'

A 0.21 5' CATTGTAACGTGGATATC 3'

M-115.6 -115591 A 0.80 5' TTCACCAAGTCAGTCTCA 3'

G 0.20 5' TTCACCAGGTCAGTCTCA 3'

M-115.4 -115397 T 0.79 5' GAGGTTTTTCTGAATACT 3'

C 0.21 5' GAGGTTTCTCTGAATACT 3'

M-115.2 -115253 A 0.79 5' TGTGGGTATCCATCATGC 3'

G 0.21 5' TGTGGGTGTCCATCATGC 3'

M-108.0 -108041 A 0.27 5' TTACAGGAACGCACCACC 3'

C 0.73 5' TTACAGGCACGCACCACC 3'

M-107.4 -107381 C 0.82 5' TGTGTTCCAGGACCAGCC 3'

A 0.18 5' TGTGTTCAAGGACCAGCC 3'

M-106.5 -106530 C 0.44 5' AAGATCACGAGCAGCTCA 3'

T 0.56 5' AAGATCATGAGCAGCTCA 3'

M-106.5a -106478 + 0.82 5' CAGGACTACATGGAATGA 3'

- 0.18 5' CACCCAGGAATGAGCACC 3'

M-106.2 -106177 G 0.77 5' CCTGCATGGCAGAGGGGG 3'

T 0.23 5' CCTGCATTGCAGAGGGGG 3'

M-104.7 -104725 C 0.28 5' GCTCACACGGTGCTGCTT 3'

T 0.72 5' GCTCACATGGTGCTGCTT 3'

M-103.4 $ -103419 T 0.97 5' CCCCCGCTGCCCCGAGAA 3'

C 0.03 5' CCCCCGCCGCCCCGAGAA 3'

M-103.3 $ -103265 T 0.78 5' CGAGGGCTGCCTGTGTGT 3'

G 0.22 5' CGAGGGCGGCCTGTGTGT 3'

M-103.1 $ -103109 A 0.84 5' AAAAGAAATGAAGCAAAT 3'

T 0.16 5' AAAAGAATTGAAGCAAAT 3'

M-102.8 $ -102838 C 0.84 5' GGTGATCCGCCTGCCTCA 3'

T 0.16 5' GGTGATCTGCCTGCCTCA 3'

M-100.7 $ -100664 T 0.84 5' AGGTGGATCTACAGGCAT 3'

A 0.16 5' AGGTGGAACTACAGGCAT 3'

M-100.5 -100442 G 0.84 5' GCCTCCAGCTCCTGGACT 3'

A 0.16 5' GCCTCCAACTCCTGGACT 3'

M-100.4 $ -100401 C 0.45 5' GACTCCTGTGCTTTTTTT 3'

T 0.55 5' GACTCCTGTGTTTTTTTT 3'

M-100.2 -100162 C 0.85 5' ATCCGCCCGCCTCAGCCT 3'

T 0.15 5' ATCCGCCTGCCTCAGCCT 3'

M-99.5 $ -99531 G 0.84 5' TCAAGCAGTTCTCCTGTC 3'

A 0.16 5' TCAAGCAATTCTCCTGTC 3'

M-99.3 -99343 T 0.79 5' ACCATGCCTGGCCTCAGT 3'

C 0.21 5' ACCACGCCTGGCCTCAGT 3'

M-98.1 $ -98093 C 0.79 5' CCAATCACATCTGCATGT 3'

T 0.21 5' CCAATCATATCTGCATGT 3'

M-98.0 $ -97976 T 0.99 5' GACAGAGTTGTGTTAACA 3'

A 0.01 5' GACAGAGATGTGTTAACA 3'

M-97.9 -97950 A 0.22 5' GGTCAGCATAGAAAATCT 3'

G 0.78 5' GGTCAGCGTAGAAAATCT 3'

M-97.8 -97759 T 0.14 5' GGGAGAGTCCCCTGCTGA 3'

C 0.86 5' GGGAGAGCCCCCTGCTGA 3'

M-97.2 -97243 A 0.51 5' TCACTTGAGGTCAGGAGT 3'

C 0.49 5' TCACTTGCGGTCAGGAGT 3'

M-96.6 -96638 C 0.59 5' GGGCATACGACATCAGGA 3'

T 0.41 5' GGGCATATGACATCAGGA 3'

M-96.1 -96063 T 0.59 5' CTCAGCTTACCGCAACCT 3'

C 0.41 5' CTCAGCTCACCGCAACCT 3'

M-96.0 $ -95980 C 0.70 5' GCCACTGCGCCCAGCTAA 3'

T 0.30 5' GCCACTGTGCCCAGCTAA 3'

M-95.8 $ -95849 C 0.69 5' TGAGCCACCACGCCCAGC 3'

T 0.31 5' TGAGCCATCACGCCCAGC 3'

M-95.3 $ -95326 A 0.57 5' CTACCACAGCAGGGCTTT 3'

T 0.43 5' CTACCACTGCAGGGCTTT 3'

M-95.0 $ -94975 A 0.51 5' TGGAAAGATGAGGCAGGC 3'

C 0.49 5' TGGAAAGCTGAGGCAGGC 3'

M-94.5 $ -94486 C 0.64 5' GGCTCAGCGATCACTCCG 3'

T 0.36 5' GGCTCAGTGATCACTCCG 3'

M-94.0 $ -93951 G 0.58 5' TGGAGACGTTTTACTGTT 3'

A 0.42 5' TGGAGACATTTTACTGTT 3'

M-85.7 -85719 G 0.94 5' TGATCTCGAACTCCTGAC 3'

A 0.06 5' TGATCTCAAACTCCTGAC 3'

M-85.2 -85215 C 0.95 5' GGCTTGGCTGGCATTCAT 3'

A 0.05 5' GGCTTGGATGGCATTCAT 3'

M-83.4 -83360 A 0.56 5' GGACCCAACTTTCCCTTC 3'

G 0.44 5' GGACCCAGCTTTCCCTTC 3'

M-83.0 -83025 T 0.56 5' CACCCCCTCAACTCTCTC 3'

C 0.44 5' CACCCCCCCAACTCTCTC 3'

M-81.7 -81703 T 0.13 5' AGCTACTTGGGAGACTGA 3'

C 0.87 5' AGCTACTCGGGAGACTGA 3'

M-80.7 -80673 A 0.56 5' GTGGCTGACCCATCCTCA 3'

G 0.44 5' GTGGCTGGCCCATCCTCA 3'

M-80.4 -80434 G 0.94 5' CCCCAAGGCTACTAATTA 3'

C 0.06 5' CCCCAAGCCTACTAATTA 3'

M-79.9 -79962 C 0.94 5' TCTGCAGCAAAAATTTTT 3'

G 0.06 5' TCTGCAGGAAAAATTTTT 3'

M-67.8 -67760 G 0.96 5' GGCTGCCGTGGTGGGTCA 3'

A 0.04 5' GGCTGCCATGGTGGGTCA 3'

M-67.4 -67397 G 0.68 5' TGCGAACGCTTCTGCAGG 3'

C 0.32 5' TGCGAACCCTTCTGCAGG 3'

M-65.4 -65361 A 0.70 5' GATATCTAGGCTTCCTAA 3'

G 0.30 5' GATATCTGGGCTTCCTAA 3'

M-61.6 -61556 T 0.65 5' GCTGCCATCCGGGGATCA 3'

C 0.35 5' GCTGCCACCCGGGGATCA 3'

M-61.5 -61523 G 0.70 5' ACTTTTCGTTGGGTTCCA 3'

T 0.30 5' ACTTTTCTTTGGGTTCCA 3'

M-61.4 -61428 C 0.96 5' ATCATTACTCCGGGGAAC 3'

G 0.04 5' ATCATTACTCGGGGGAAC 3'

M-61.4a $ -61420 A 0.74 5' GGGGAACACTGTGAAGTT 3'

T 0.26 5' GGGGAACTCTGTGAAGTT 3'

M-61.2 -61184 C 0.96 5' CTGGCTGCCAAACACAAA 3'

T 0.04 5' CTGGCTGTCAAACACAAA 3'

M-60.8 -60755 T 0.67 5' TAGTCAATTTTTGCAAAG 3'

A 0.33 5' TAGTCAAATTTTGCAAAG 3'

M-60.0 $ -60011 C 0.13 5' TGCTCAACTGTAACCACG 3'

T 0.87 5' TGCTCAATTGTAACCACG 3'

M-59.7 $ -59698 G 0.74 5' AATTCCAGCTACTCAGGA 3'

C 0.26 5' AATTCCACCTACTCAGGA 3'

M-59.6 $ -59574 - 0.74 5' AAAAAAAAAACATAGGTC 3'

+ 0.26 5' AAAAAACAAACATAGGTC 3'

M-58.9 -58933 T 0.74 5' AGCCCGGTGGGCTCTCTC 3'

G 0.26 5' AGCCCGGGGGGCTCTCTC 3'

M-58.3 $ -58274 T 0.54 5' GTGTAACTATATATGTAA 3'

C 0.46 5' GTGTAACCATATATGTAA 3'

M-57.8 -57837 T 0.49 5' AATCACCTCCCACCCCAC 3'

C 0.51 5' AATCACCCCCCACCCCAC 3'

M-57.1 -57099 G 0.62 5' AAGGGTGGAGACAGGATG 3'

A 0.38 5' AAGGGTGAAGACAGGATG 3'

M-57.0 -57039 G 0.78 5' TCTGGGGGAGATGACTCC 3'

C 0.22 5' TCTGGGGCAGATGACTCC 3'

M-56.9 -56845 A 0.78 5' CGTCCACAGTTWGGGATA 3'

C 0.22 5' CGTCCACCGTTWGGGATA 3'

M-56.8 -56841 T 0.62 5' CACNGTTTGGGATACTCG 3'

A 0.38 5' CACNGTTAGGGATACTCG 3'

M-56.6 -56631 G 0.61 5' GGATTCCGTATCAAACTA 3'

A 0.39 5' GGATTCCATATCAAACTA 3'

M-55.5 -55481 C 0.62 5' GGCTCCACGGCTGTGAGA 3'

T 0.38 5' GGCTCCATGGCTGTGAGA 3'

M-55.5a -55452 C 0.78 5' ACTTTCCCAAGCTCTTAT 3'

T 0.22 5' ACTTTCCTAAGCTCTTAT 3'

M-55.4 -55421 T 0.62 5' CATGTAATGCTCACAACT 3'

C 0.38 5' CATGTAACGCTCACAACT 3'

M-54.5 -54462 A 0.61 5' ATTTCTGAAGTGATAAGA 3'

G 0.39 5' ATTTCTGGAGTGATAAGA 3'

M-53.2 -53184 G 0.61 5' TTATACGGTGGACAGAGG 3'

A 0.39 5' TTATACGATGGACAGAGG 3'

M-51.5 -51518 C 0.61 5' AAAATATCGATAGATTTT 3'

G 0.39 5' AAAATATGGATAGATTTT 3'

M-50.7 -50659 C 0.84 5' GAGAGGACAGCCTGTGCA 3'

T 0.16 5' GAGAGGATAGCCTGTGCA 3'

M-50.4 -50350 T 0.60 5' ATGCATCTTTTTTGGAGC 3'

C 0.40 5' ATGCATCCTTTTTGGAGC 3'

M-50.0 -49980 G 0.61 5' TCAAGGTGTATCTTGTAG 3'

C 0.39 5' TCAAGGTCTATCTTGTAG 3'

M-49.2 -49235 C 0.84 5' TGGGCAACAGAGCAAGAC 3'

A 0.16 5' TGGGCAAAAGAGCAAGAC 3'

M-47.6 -47643 A 0.76 5' ATATAGCATCAAGAAGCA 3'

G 0.24 5' ATATAGCGTCAAGAAGCA 3'

M-31.9 -31861 G 0.84 5' CCGGGAGGGGGAGCAGAG 3'

A 0.16 5' CCGGGAGAGGGAGCAGAG 3'

M-31.8 -31756 T 0.76 5' CACCCCATTGCCGCTCGG 3'

C 0.24 5' CACCCCACTGCCGCTCGG 3'

M-29.7 -29732 G 0.59 5' AGTTTTCGTGATTCTTAT 3'

A 0.41 5' AGTTTTCATGATTCTTAT 3'

M-29.6 -29642 G 0.58 5' AGGCACAGTCCTTTCTTT 3'

A 0.42 5' AGGCACAATCCTTTCTTT 3'

A2 $ -11022 T 0.42 5’ CCTGGCATCATGGTTTGT 3’

A 0.58 5’ CCTGGCAACATGGTTTGT 3’

A4 $ -10628 T 0.40 5’ AAAGGTTTTGGCTTTAAA 3’

C 0.60 5’ AAAGGTTCTGGCTTTAAA 3’

A6 $ -8640 G 0.41 5’ AAGCTAGGGTGTGGAACG 3’

A 0.59 5’ AAGCTAGAGTGTGGAACG 3’

A8 $ -6468 C 0.60 5’ GAAACTACAATGATCGGC 3’

A 0.40 5’ GAAACTAAAATGATCGGC 3’

A10 $ -5508 A 0.60 5’ GAGAGGAAGTGAACAACA 3’

C 0.40 5’ GAGAGGACGTGAACAACA 3’

Al $ -3592 T 0.41 5' TGTGGACTGCTTTGTAGC 3'

A 0.59 5' TGTGGACAGCTTTGTAGC 3'

Ba $ -3382 T 0.40 5' TGTTGTTACTTGTATGTG 3'

G 0.60 5' TGTTGTTACGTGTATGTG 3'

Rs $ -3373 T 0.78 5' GTATGTGTACTTCATTCT 3'

G 0.22 5' GTATGTGGACTTCATTCT 3'

Bc $ -3058 T 0.59 5' GCCAACATGATCAGTCTT 3'

C 0.41 5' GCCAACACGATCAGTCTT 3'

Xb $ -2914 C 0.40 5' CCCAGTCCAGAGTGCAGT 3'

T 0.60 5' CCCAGTCTAGAGTGCAGT 3'

Aw $ -2590 T 0.40 5' GAAGTCTTGCTGTGTGTC 3'

C 0.60 5' GAAGTCTCGCTGTGTGTC 3'

Bs $ -2143 A 0.41 5' ATTCAGAATGCAACTCCT 3'

G 0.59 5' ATTCAGAGTGCAACTCCT 3'

Rt $ -1125 A 0.78 5' ATCTCAAATACAATTAAA 3'

G 0.22 5' ATCTCAAGTACAATTAAA 3'

M1 $ -971 C 0.46 5' GCCATACCCTTCATTTTT 3'

T 0.54 5' GCCATACTCTTCATTTTT 3'

Sa $ -721 C 0.79 5' ACTTCATCCACAAAGCAG 3'

T 0.21 5' ACTTCATTCACAAAGCAG 3'

H1 $ -441 G 0.82 5' AGAAATAGCACTCAAACA 3'

C 0.17 5' AGAAATACCACTCAAACA 3'

Hf $ -378 + 0.79 5' TGCGACTCGCAGATGGAG 3'

- 0.21 5' TGCGACTTGCAGATGGAG 3'

H2 $ -280 C 0.63 5' CCCGTATCGGCTAGGGTT 3'

T 0.38 5' CCCGTATTGGCTAGGGTT 3'

O1 $ -187 G 1.00 5' GTAGTTTGGTGGGAAGGG 3'

C 0.00 5' GTAGTTTCGTGGGAAGGG 3'

O2 $ -177 G 1.00 5' GGGAAGGGTGGTTACAGA 3'

C 0.00 5' GGGAAGGCTGGTTACAGA 3'

Hp $ 1006 C 0.62 5' TTGAACCCGGGAGGCGGA 3'

T 0.38 5' TTGAACCTGGGAGGCGGA 3'

Bb $ 1347 C 0.23 5' AGGGAGACGATAAAGAAA 3'

G 0.77 5' AGGGAGAGGATAAAGAAA 3'

Sf $ 2195 G 0.81 5' TTGGACAGGCTGGTCTTG 3'

A 0.19 5' TTGGACAAGCTGGTCTTG 3'

Ec $ 2879 G 0.79 5' CTCGGCAGGGTCCCTTCT 3'

A 0.21 5' CTCGGCAAGGTCCCTTCT 3'

M+3.7 3671 G 0.77 5' CAGGCCCGTAATTCACCC 3'

A 0.23 5' CAGGCCCATAATTCACCC 3'

Bl $ 5123 C 0.21 5' GGACAACCCAGCGTCCGG 3'

A 0.79 5' GGACAACACAGCGTCCGG 3'

M+5.4 5389 G 0.77 5' TGGGGGTGTTTTCTCTCC 3'

A 0.23 5' TGGGGGTATTTTCTCTCC 3'

M+10.4 10449 G 0.99 5' TATCTGTGTTAAAAATAA 3'

A 0.01 5' TATCTGTATTAAAAATAA 3'

M+11.8 11831 A 0.78 5' AGGTCAGGAGTTCAAGAC 3'

G 0.22 5' AGGTCGGGAGTTCAAGAC 3'

M+12.2 12169 C 0.79 5' TCAGCAGCCACATGGGGC 3'

T 0.21 5' TCAGCAGTCACATGGGGC 3'

M+12.4 12447 G 0.79 5' ACTTTGCGGATGAGGAAA 3'

A 0.21 5' ACTTTGCAGATGAGGAAA 3'

M+13.2 13152 G 0.82 5' CCATGCCGTCCACCATCC 3'

A 0.18 5' CCATGCCATCCACCATCC 3'

M+13.6 13605 G 0.61 5' GCCTGGCGTAGACTCCAT 3'

A 0.39 5' GCCTGGCATAGACTCCAT 3'

M+14.3 14263 G 0.82 5' TCGTCACGTCCCCAGAGA 3'

A 0.17 5' TCGTCACATCCCCAGAGA 3'

M+14.4 14384 C 0.61 5' AGACGACCTTCCCCAAGA 3'

T 0.39 5' AGACGACTTTCCCCAAGA 3'

M+14.5 14535 C 0.60 5' GAGGACCCGCAGATACTC 3'

T 0.40 5' GAGGACCTGCAGATACTC 3'

M+14.7 $ 14702 + 0.60 5' CCAACATTGAAAACACCC 3'

- 0.40 5' CAGTGCCGTCACCCAGGA 3'

M+14.8 $ 14838 A 0.60 5' TGGGGCCAGGCCATCATC 3'

G 0.40 5' TGGGGCCGGGCCATCATC 3'

M+14.9 14923 A 0.79 5' CTGACCCAGGCGACTACA 3'

G 0.21 5' CTGACCCGGGCGACTACA 3'

M+15.5 15529 C 0.57 5' ATTAAAACGGCAGATTCA 3'

A 0.43 5' ATTAAAAAGGCAGATTCA 3'

M+15.6 * 15589 A 0.42 5' ATCGCACATAACACCACA 3'

G 0.58 5' ATCGCACGTAACACCACA 3'

M+15.8 * 15837 C 0.69 5' CCACACGCAGTTACCCTG 3'

T 0.31 5' CCACACGTAGTTACCCTG 3'

M+16.2 16213 T 0.80 5' ACCTGCCTCATGAGCCAC 3'

C 0.20 5' ACCTGCCCCATGAGCCAC 3'

M+16.3 * 16246 G 0.75 5' CTATTCCGACCTTACACA 3'

A 0.25 5' CTATTCCAACCTTACACA 3'

M+16.6 * 16638 G 0.60 5' TAGATGTGTCACTCCAGT 3'

A 0.40 5' TAGATGTATCACTCCAGT 3'

M+16.7 16694 G 0.80 5' TCTGTCTGTGTCCAAACA 3'

A 0.20 5' TCTGTCTATGTCCAAACA 3'

M+16.9 * 16936 T 0.77 5' GCCAAGATCTGCAGCTTG 3'

A 0.23 5' GCCAAGAACTGCAGCTTG 3'

M+17.0 17008 A 0.80 5' CCTGTGCAAAGGCCCTGG 3'

G 0.20 5' CCTGTGCGAAGGCCCTGG 3'

M+17.2 17247 G 0.80 5' CCATCACGTATCATTCAG 3'

A 0.20 5' CCATCACATATCATTCAG 3'

M+17.4 17371 T 0.80 5' GTCCACATTCCCCACTGA 3'

C 0.20 5' GTCCACACTCCCCACTGA 3'

M+17.7 17718 T 0.08 5' CGCCTGTTAYCCCAGCAC 3'

C 0.92 5' CGCCTGTCAYCCCAGCAC 3'

M+17.7a 17720 C 0.81 5' CCTGTYACCCCAGCACTT 3'

T 0.19 5' CCTGTYATCCCAGCACTT 3'

M+18.0 18040 T 0.80 5' AGCCCAGTGAGGTCGAGG 3'

G 0.20 5' AGCCCAGGGAGGTCGAGG 3'

M+18.5 $ 18486 A 0.65 5' GAACTCCATCTCAGAGAA 3'

G 0.35 5' GAACTCCGTCTCAGAGAA 3'

M+18.7 * 18679 C 0.92 5' GCGTGATCTTGGCTCAGC 3'

T 0.08 5' GCGTGATTTTGGCTCAGC 3'

M+18.7a * 18747 G 0.98 5' GTAGCTGGGATTACAGGC 3'

A 0.02 5' GTAGCTGAGATTACAGGC 3'

M+19.5 19549 G 0.80 5' CAGGAATGCATATCTGCT3'

T 0.20 5' CAGGAATTCATATCTGCT3'

M+19.6 19641 G 0.81 5' GTTATTTGAAAGTCACAT 3'

A 0.19 5' GTTATTTAAAAGTCACAT 3'

M+19.9 * 19957 C 0.75 5' CTGTCTCCARAAAGAAAA 3'

A 0.25 5' CTGTCTCAARAAAGAAAA 3'

M+19.9a * 19959 G 0.75 5' CTGTCTCMAGAAAGAAAA 3'

A 0.25 5' CTGTCTCMAAAAAGAAAA 3'

M+20.0 19977 T 0.80 5' AAAAGTGTCAAGTTGAGA 3'

A 0.20 5' AAAAGTGACAAGTTGAGA 3'

M+20.0a * 20011 G 0.75 5' TATTTTTGAGATGGAGTC 3'

C 0.25 5' TATTTTTCAGATGGAGTC 3'

M+20.1 20061 C 0.81 5' TGGCACCATCTCGGCTCA 3'

T 0.19 5' TGGCACCATCTTGGCTCA 3'

M+20.2 20151 T 0.81 5' CACCTCGTCCGGGTAATT 3'

C 0.19 5' CACCTCGCCCGGGTAATT 3'

M+20.3 20259 T 0.81 5' TGCTGGGATTACAGGTGT 3'

C 0.19 5' TGCCGGGATTACAGGTGT 3'

M+20.4 20423 A 0.81 5' TGATGGTAGTCTACCTCC 3'

G 0.19 5' TGATGGTGGTCTACCTCC 3'

M+20.7 $ 20758 G 0.64 5' TTCAGCACGAGCYCTCCT 3'

A 0.36 5' TTCAGCACAAGCYCTCCT 3'

M+20.8 $ 20768 T 0.64 5' CTCCTTCTTAGCCTTCTA 3'

C 0.36 5' CTCCTCCTTAGCCTTCTA 3'

M+20.9 $ 20913 T 0.55 5' AGTGGGGTGATATACATA 3'

C 0.45 5' AGTGGGGCGATATACATA 3'

M+21.2 $ 21227 G 0.43 5' AGGCCTCCTGGGGGCTGA 3'

A 0.57 5' AGGCCTCCTGAGGGCTGA 3'

M+21.2a $ 21235 G 0.42 5' GGGCTGAGTTTCATGGGC 3'

A 0.58 5' GGGCTGAATTTCATGGGC 3'

M+21.4 $ 21411 T 0.86 5' GTTTTTCTGTTTGATTGT 3'

G 0.14 5' GTTTTTCGGTTTGATTGT 3'

M+21.5 $ 21502 T 0.91 5' CTAGAACTTATGATGTAG 3'

C 0.09 5' CTAGAACTTACGATGTAG 3'

M+21.5a * 21552 G 0.87 5' GCAAGGTCCTGGAAGCAG 3'

C 0.13 5' GCAAGGTCCTCGAAGCAG 3'

M+21.5b $ 21560 A 0.40 5' GAAGCAGAGGTCGGGGGC 3'

G 0.60 5' GAAGCAGGGGTCGGGGGC 3'

M+21.6 $ 21573 G 0.87 5' GGTCGGGGGCGTGGGGGA 3'

C 0.13 5' GGTCGGGGGCGTCGGGGA 3'

M+21.9 $ 21879 G 0.63 5' GACCTCAGTTGATCTGCG 3'

C 0.37 5' GACCTCACTTGATCTGCG 3'

M+21.9a $ 21944 C 0.40 5' CTGTGCCTGGCCCCTTTT 3'

T 0.60 5' CTGTGCCTGGCCTCTTTT 3'

M+22.0 $ 21971 G 0.91 5' TTATGTTTTGAGACGGAG 3'

A 0.09 5' TTATGTTTTGAGACAGAG 3'

M+22.1 $ 22055 T 0.42 5' ATTTTCCTGCCTCAGCCT 3'

C 0.58 5' TGGCTCAAGCGATTCTCC 3'

M+22.1a * 22115 T 0.35 5' CCCGGCCTCTTTTCATGT 3'

G 0.65 5' CCCGGCCTCTTGTCATGT 3'

M+22.3 $ 22308 G 0.51 5' CTGGTTTGCCTGCCTCTG 3'

C 0.49 5' CTGGTTTCCCTGCCTCTG 3'

M+22.8 $ 22781 G 0.93 5' GTCTCGATGGAGTGAAAA 3'

A 0.07 5' GTCTCGATGGAATGAAAA 3'

M+23.8 $ 23758 G 0.75 5' TTCCTTTGACAAACCCAG 3'

T 0.25 5' TTCCTTTTACAAACCCAG 3'

M+23.9 $ 23902 G 0.80 5' CTGAGCTGGTGGTTGCAG 3'

A 0.20 5' CTGAGCTAGTGGTTGCAG 3'

M+23.9a $ 23938 C 0.99 5' CCAGCTTCGCCCCCCGGG 3'

T 0.01 5' CCAGCTTTGCCCCCCGGG 3'

M+24.0 $ 23953 G 0.74 5' GGGAGATGTGCCACCCCT 3'

A 0.26 5' GGGAGATATGCCACCCCT 3'

M+24.4 $ 24436 G 0.99 5' GCCTCCCGAGTAGCTGGG 3'

A 0.01 5' GCCTCCCAAGTAGCTGGG 3'

M+25.0 25020 G 0.94 5' CTCATTTGAGTATCTACC 3'

T 0.06 5' CTCATTTTAGTATCTACC 3'

M+25.5 25528 C 0.56 5' GTGACAACGTTCCCTTGC 3'

T 0.44 5' GTGACAATGTTCCCTTGC 3'

M+25.6 * 25618 T 0.54 5' TATTTTTTTTTTTTATTT 3'

A 0.46 5' TATTTTTATTTTTTATTT 3'

M+27.3 $ 27301 T 0.29 5' GTGCACATTACCATGCTA 3'

C 0.71 5' GTGCACACTACCATGCTA 3'

M+27.5 27563 G 0.49 5' ATTCCACGTGGTTCTGGC 3'

C 0.51 5' ATTCCACCTGGTTCTGGC 3'

M+28.2 $ 28245 - 0.49 5' ACCTCTAGTCTGATGTAG 3'

+ 0.51 5' ACCTCTAGTCCCAGCTAC 3'

M+28.6 $ 28621 G 0.96 5' TACAAACGCTCTTTCCTG 3'

C 0.04 5' TACAAACCCTCTTTCCTG 3'

M+29.1 29055 T 0.29 5' TGGAGTGTAGTGGCTCAA 3'

C 0.71 5' TGGAGTGCAGTGGCTCAA 3'

M+29.2 29158 C 0.51 5' CTGCCACCATGCCTGGCT 3'

T 0.49 5' CTGCCACCATGTCTGGCT 3'

M+30.1 $ 30109 G 0.78 5' ATTGGCTGTTGTCATCCC 3'

C 0.22 5' ATTGGCTCTTGTCATCCC 3'

M+30.2 30164 C 0.56 5' TGGGCCACGTTGCCCTGT 3'

T 0.44 5' TGGGCCATGTTGCCCTGT 3'

M+30.5 $ 30490 G 0.28 5' GCTCTCAGTCCTCGCAGC 3'

A 0.72 5' GCTCTCAATCCTCGCAGC 3'

M+30.8 $ 30765 G 0.74 5' TCGCTCCGTTGCCCAGGC 3'

A 0.26 5' TCGCTCCATTGCCCAGGC 3'

M+31.1 $ 31080 G 0.28 5' AGGCTGGGCTCGAACTTC 3'

T 0.72 5' AGGCTGGTCTCGAACTTC 3'

M+31.9 $ 31941 A 0.55 5' CAGTATGAGATCCAGCAT 3'

G 0.45 5' CAGTATGGGATCCAGCAT 3'

M+32.0 $ 31973 C 0.80 5' TTGCAATCCCTGCTGTTT 3'

G 0.20 5' TTGCAATGCCTGCTGTTT 3'

M+32.1 $ 32075 T 0.50 5' ACTCTAGTAGGGTCCGTC 3'

C 0.50 5' ACTCTAGCAGGGTCCGTC 3'

M+32.1a $ 32126 C 0.56 5' TGCTGGCCAAATCCTACT 3'

T 0.44 5' TGCTGGCTAAATCCTACT 3'

M+32.3 32309 C 0.77 5' GGTCTTTCTCCCCTCTCT 3'

T 0.23 5' GGTCTTTCTTCCCTCTCT 3'

M+32.3a 32318 G 0.77 5' CCTCTCTGAGCACAAAGC 3'

A 0.23 5' CCTCTCTAAGCACAAAGC 3'

M+32.8 32808 C 0.46 5' ATTCTTTCTGTTGTCATT 3'

T 0.54 5' ATTCTTTTTGTTGTCATT 3'

M+32.9 32889 G 0.51 5' AGGCTGGAGTGCCGTGGC 3'

A 0.49 5' AGGCTGGAGTGCCATGGC 3'

M+32.9a 32894 A 0.51 5' TGGCATGATTATAGCTCA 3'

G 0.49 5' TGGCGTGATTATAGCTCA 3'

M+33.2 33212 G 0.48 5' TTAAAGAGGATCTCTAGT 3'

A 0.52 5' TTAAAGAAGATCTCTAGT 3'

M+34.3 34283 C 0.51 5' TTGTCCTCGATTTGGAGT 3'

A 0.49 5' TTGTCCTAGATTTGGAGT 3'

M+34.5 34534 T 0.46 5' GAGAGCATAAAATAGGCA 3'

A 0.54 5' GAGAGCAAAAAATAGGCA 3'

M+34.6 34561 C 0.51 5' CGTATATCTTAGAAAAAG 3'

T 0.49 5' CGTATATTTTAGAAAAAG 3'

M+35.3 35291 A 0.50 5' ACAAAGTATTCCTTCCCA 3'

G 0.50 5' ACAAAGTGTTCCTTCCCA 3'

M+35.5 35474 C 0.91 5' CACCAAGCCTTGCAACTA 3'

G 0.09 5' CACCAAGGCTTGCAACTA 3'

M+35.5a 35537 A 0.51 5' AATACAAATCAGATCTTA 3'

G 0.49 5' AATACAAGTCAGATCTTA 3'

M+35.7 35664 C 0.66 5' TCCTGAGCAGTATAGAGG 3'

T 0.34 5' TCCTGAGTAGTATAGAGG 3'

M+35.8 35780 A 0.86 5' TCTTTTCACTCCCTAAAT 3'

G 0.14 5' TCTTTTCGCTCCCTAAAT 3'

M+35.8a 35818 T 0.67 5' GAATTCCTGAGGACAGAT 3'

C 0.33 5' GAATTCCCGAGGACAGAT 3'

M+37.6 37616 A 0.38 5' TCAATGAAGATGCTAAAA 3'

C 0.62 5' TCAATGACGATGCTAAAA 3'

M+38.3 38303 A 0.97 5' CCAACATATCCCTCATTT 3'

T 0.03 5' CCAACATTTCCCTCATTT 3'

Last updated: 1 September, 2005
Celia A. May
The views expressed in this document are those of the document owner, Alec J. Jeffreys.