College of Medicine, Biological Sciences and Psychology
Advanced Imaging Facilities

Microscopy workshops 2012

20th - 24th August 2012

From basic to advanced microscopy

After a year of absence we will run this year again two fluorescence workshops during the week of 20-24 of August. In the past these workshops have been very successful. The first day will be an Immunolabelling workshop with lectures and hands-on sessions. During this day the participants will do an Immunolabelling. This day is followed by a 4 day workshop called 'Fluorescence microscopy and quantitative imaging.

For full program click here.

Payment for the workshop and/or accommodation is taken care of by Shop@le.
At the moment only a few places are left. Please send me an email (krs5@le.ac.uk) before you sign in for the workshop to avoid disappointment; Deadline Monday 13 August 2012.

Download the workshop poster.

workshop overview:
20th August 2012: Immunolabelling
This workshop will give an introduction to immunolabelling with lectures and hands-on sessions. The participants will perform an immunolabelling using a single cell system. During lectures sample preparation, fixation, blocking, choice of antibodies, mounting medium and other relevant topics will be discussed. Participants who want to bring their own samples and antibodies for immunolabelling please contact Dr. Kees Straatman

21st - 24th August 2012: Fluorescence microscopy and quantitative imaging
This workshop will cover basic microscopy and fluorescence microscopy before introducing more advanced techniques like FRAP, FLIP and FRET. The participants will be introduced to different types of fluorescence microscopes, like confocal laser scanning microscopy and multi photon scanning microscope as well as image analysis software in lectures and hands-on sessions. We will also have a look at the latest developments in fluorescence microscopy.
Participants are welcome to bring their own samples to view on the different microscope systems available during the workshop or image files for analysis using any of the available software packages during the workshop. There is also the option to send own material in advance to do a small test screen on your material. Please contact Dr. Kees Straatman to discuss this option.

Confirmed equipment available during this workshop for hands-on sessions:
(not complete yet)
- Olympus FV1000 confocal laser scanning microscope
- Olympus ScanR system
- Olympus TIRFM system
- Olympus Kohler/DIC/Phase contrast
- Leica TCS SP5 confocal laser scanning microscope
- Zeiss LSM7 multiphoton laser scanning microscope
- Andor Revolution® XD spinning disk laser microscopy system
- Imaris, the cutting-Edge 3D and 4D Imaging Software for Life Sciences from Bitplane
- Volocity, PerkinElmer's high performance 3D imaging software package

Workshop fees:

Fees include lecture printouts, consumables for immunolabelling workshop, coffee, lunches and a dinner during the fluorescence microscopy workshop. Drinks and dinner are sponsored by Olympus.

Where are the workshops:
The immunolabelling workshop will be given in the Adrian Building. The Fluorescence microscopy workshop lectures will be given in the Adrian Building and hands-on sessions in the Adrian Building, Henry Wellcome Building and the Maurice Shock Building (subject to changes). All buildings are in close proximity to each other. See map.

Who should attend:
These workshops will give an overview of different techniques and microscope systems and are aimed at anyone who:
- is new to fluorescence microscopy
- wants to gain hands-on experience of modern fluorescence techniques
- wants to analyse and quantify his/her imaging data
- wants to refresh their knowledge
- wants to make better use of fluorescence microscopy
- uses fluorescence microscopy and wants to improve his/her knowledge
- uses basic fluorescence microscopy and wants to use more advanced techniques
- students (PhD, MSc), postdocs, researchers and clinical researchers

Where to stay:
During the workshop a limited number of rooms are available from the University Accommodation Service at Opal Court (see: http://www2.le.ac.uk/offices/accommodation/student-accommodation/self-catered-city-living/opal-court-1) on a first come first served basis. Opel Court is a few minutes walk from the workshop location. These rooms are on a self-catering base for £32 per night, en suite and hand towels are included.

Other places to stay in Leicester can be found here or alternatively here.